This work investigated the metabolites and their biosynthetic functional hydroxylase genes of the deep-sea sediment metagenomic clone 25D7.5-Bromoindole was added to the 25D7 clone derived Escherichia coli fermentation broth.The new-generated metabolites and their biosynthetic byproducts were located through LC-MS, in which the isotope peaks of brominated products emerged.Two new brominated bis-indole metabolites, 5-bromometagenediindole B (1), and 5-bromometagenediindole UHF TV Antenna C (2) were separated under the guidance of LC-MS.Their structures were elucidated on the basis of 1D and 2D NMR spectra (COSY, HSQC, and HMBC).
The biosynthetic Bathroom Products functional genes of the two new compounds were revealed through LC-MS and transposon mutagenesis analysis.5-Bromometagenediindole B (1) also demonstrated moderately cytotoxic activity against MCF7, B16, CNE2, Bel7402, and HT1080 tumor cell lines in vitro.